Non-specific fluorescence background can have several causes, including sample autofluorescence, non-specific antibody binding, and electrostatic interactions of fluorescent dyes with proteins and blotting membranes. TrueBlack® reagents for IF staining and western blotting block background from multiple sources for optimal specificity.
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Mini Syringe Filters and Mini Syringe Filtration Kit
These sterile 0.22 um filters are useful for filtering 100 uL to 1 mL of aqueous solutions with only 10 uL hold-up volume.
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Paraformaldehyde, 4% in PBS, Ready-to-Use Fixative
A ready-to-use fixation solution for cells or tissues. Electron microscopy-grade, methanol-free paraformaldehyde, packaged under argon.
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TCEP
TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.
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TrueBlack® IF Background Suppressor System (Permeabilizing)
The TrueBlack® IF Background Suppressor System is a buffer system designed for optimal blocking of non-specific staining for immunofluorescence (IF).
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TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF
TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF, is a novel reagent that quenches lipofuscin autofluorescence in tissue sections for immunofluorescence staining.
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TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO
Quench lipofuscin autofluorescence in PBS, with minimal background.
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TrueBlack® WB Blocking Buffer Kit
Buffer system optimized for fluorescence-based western provides superior specificity, sensitivity, and background suppression.
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Water, Ultrapure Molecular Biology Grade
Suitable for use in molecular biology applications that require high quality, ultra-pure water.
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