MasterPure Yeast RNA Purification Kit

Safe, fast purification of high quality RNA from multiple species of yeast.

Key features

  • Safe, fast purification of high quality RNA from multiple species of yeast
  • Safe: Avoids the use of dangerous hot acid phenol
  • Fast: Quicker protocol that methods using spheroplasting
  • High Quality RNA: RNA quality exceeds that obtained by bead beating protocols and is ready for use in downstream applications such and RT-PCR and microarray analysis
  • Excellent Yields: Yields 25-50 µg RNA from 1 ml of mid-log S.cerevisiae
  • Complete: All the necessary reagents are supplied with no need to purchase extra equipment
Name SKU Size Availability Vendor Price Order
MasterPure Yeast RNA Purification Kit 100 Purifications Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Predict Details
Contents: Extraction Reagent for RNA, MPC Protein Precipitation Reagent, TE Buffer (in 100-reaction kit only), Proteinase K, RNase-Free DNase I, RiboGuard RNase Inhibitor, 10X DNase Buffer, 2X T & C Lysis Solution.

Product information
The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi. The kit uses a rapid salting-out process1 to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.2

References

  1. Miller, S.A. et al., (1988) Nucleic Acids Res. 16, 1215.
  2. Hoffman, L.M. and Jarvis, B.W. (2004) Epicentre Forum 11(2), 4.

 

Epicentre-g_masterpureyeast_purifiedyeast Epicentre-c_masterpureyeast_rnapurity
Figure 1. Microarray analysis of yeast RNA purified with the MasterPure™ Yeast RNA Purification Kit. The RNA was reverse transcribed, labelled, and hybridised to a S. cerevisiae DNA microarray. The cDNA from cells under oxidative stress was labelled with Cy5, and cDNA from control cells was labelled with Cy3. Figure 2. Purity of RNA obtained using the MasterPure™ Yeast RNA Purification Kit. The RNA used in Figure. 1. was stored at -20 °C for two months before analysis on an Agilent Technologies 2100 Bioanalyzer®. The electrophoretogram demonstrates the high-purity, intact RNA.

Resources

SDS

Manuals and User Guides

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