PerfeCTa FastMix II

Features

PerfeCTa FastMix II is an advanced 2X concentrated qPCR SuperMix reagent for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan hydrolysis probes. The kit is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. Inert AccuVue plate loading dye helps to minimize pipette error and provides visual confirmation of thorough mixing. A key component of the kit is an ultra pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA. PerfeCTa FastMix II is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples.

Features & Benefits

• 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy
• Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot-start
• Inert AccuVue plate loading dye simplifies reaction setup and provides instant visual cue of reagent addition and mixing
• Supports efficient vortex mixing with proprietary anti-foaming technology
• Flexible – optimized for both fast and standard thermal cycling conditions

Contents

Single-tube, 2X concentrated reagent containing:

• Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
• AccuStart II Taq DNA Polymerase.
• Inert AccuVue dye.
• Proprietary enzyme stabilizers and performance-enhancing additives.
• Titrated reference dye (if applicable).

Storage & Handling

• Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
• Repeated freezing and thawing does not affect reagent performance.
• After thawing, mix thoroughly before using.
• For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

PerfeCTa FastMix II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.