PerfeCTa qPCR FastMix UNG

A 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR systems that do not require an internal reference dye

Name SKU Size Availability Vendor Price Order

Perfecta qPCR FastMix UNG
1250 x 20 μL rxns (10 x 1.25 mL)

 95076-012 1250 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Perfecta qPCR FastMix UNG
5000 x 20 μL rxns (1 x 50 mL)

 95076-05K 5000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Perfecta qPCR FastMix UNG ROX
1250 x 20 μL rxns (10 x 1.25 mL)

 95077-012 1250 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Perfecta qPCR FastMix UNG ROX
5000 x 20 μL rxns (1 x 50 mL)

 95077-05K 5000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Perfecta qPCR FastMix UNG Low-ROX
1250 x 20 μL rxns (10 x 1.25 mL)

 95078-012 1250 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Perfecta qPCR FastMix UNG Low-ROX
5000 x 20 μL rxns (1 x 50 mL)

 95078-05K 5000 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Photos

Features

PerfeCTa qPCR FastMix, UNG is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR systems that do not require an internal reference dye. The proprietary buffer and stabilizers have been specifically optimized to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal with TaqMan® or TaqMan MGB probe chemistry when using rapid PCR cycle times and reduced reaction volumes. This affords greater reagent economy and laboratory throughput on conventional or rapid ramp rate qPCR systems. The enhanced specificity of this FastMix suppresses cross-reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component of this FastMix is AccuFast Taq DNA polymerase. This hot-start Taq contains a proprietary mixture of monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step (> 48 hours at room temperature). Similar to our AccuStart™ Taq DNA polymerase, these antibodies are irreversibly inactivated during the initial PCR denaturation step. However, unlike other antibody hot-start polymerases, activation of AccuFast Taq is instantaneous at 95ºC. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200 bp is complete in less than 20s at 60ºC. Additionally, the dNTP mix in this FastMix contains dUTP in place of dTTP. Inclusion of uracil-N-glycosylase (UNG) prevents amplification of carry-over contamination from previous dU-containing PCRs.

Features & Benefits

  • Fast Cycling protocols
  • contains UNG to eliminate amplification of carry-over contamination

PerfeCTa qPCR FastMix UNG Kits are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Contents

  • 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs, AccuFast Taq DNA Polymerase, UNG, and stabilizers

Storage & Handling

  • Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
  • For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Resources

Product Manuals

CofA (PSFs)

SDSs

FAQs

Publications

TIRAP/Mal Positively Regulates TLR8‐Mediated Signaling via IRF5 in Human Cells
Kaja Elisabeth Nilsen – 2022
Iron oxide nanoparticles enhance Toll-like receptor-induced cytokines in a particle size- and actin-dependent manner in human blood
Susann Wolf-Grosse – 2018

You may also like

wp_reset_postdata();