qScript 1-Step Virus ToughMix

Features

qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses such as Flu-A, Flu-B, and SARS-CoV-2, using one-step, or single-tube reverse transcription quantitative PCR (RT-qPCR). It has been optimized for maximum sensitivity to enable reliable quantification of very low input quantities of RNA using dual-labeled hydrolysis probe detection chemistries such as TaqMan® probes in single or multiplexed assay formats. qScript 1-Step Virus ToughMix contains all required components for RT-qPCR except RNA template and primer/probe assays.

qScript 1-Step Virus ToughMix is powered by an engineered reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures, that includes a RT Hot-Start technology to suppresses non-specific primer extension by the RT before cDNA synthesis. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis. These features combined with a stringent mAb hot-start Taq polymerase and ToughMix reagent technology to neutralize many common PCR inhibitors, provide reproducible low-copy quantification as well as extended room temperature stability of fully assembled reactions for automated reaction assembly.

qScript 1-Step Virus ToughMix does not contain an internal reference dye.

Contents

• 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride,
• qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase,
• AccuVue blue qPCR dye, and stabilizers

Storage & Handling:

• Store components in a constant temperature freezer at -25°C to -15°C.
• Repeated freezing and thawing do not affect RT-qPCR performance.
• For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.