qScript cDNA Synthesis Kit

Economical 2-component kit ideally suited for high throughput gene-expression studies.

Name SKU Size Availability Vendor Price Order

qScript cDNA Synthesis Kit Sample Size
25 x 20 μL rxns (1 x 25 µL)

 95047-025 25 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

qScript cDNA Synthesis Kit
100 x 20 μL rxns (1 x 100 µL)

 95047-100 100 Rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

qScript cDNA Synthesis Kit
500 x 20 μL rxns (1 x 500 µL)

 95047-500 500 rxns Generally 1-2 weeks from receipt of order QuantaBio Log in for pricing

Photos

Features

The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR. The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. qScript reverse transcriptase is a mixture of an engineered MMLV RT and a ribonuclease inhibitor protein. This economical, highly stable stabilize, 2-component reagent system has been rigorously optimized to ensure sensitive and linear RNA detection with a wide-range of input RNA and relative abundance. Reagent performance is unaffected by repetitive freeze/thaw cycling (>20X), conferring greater ease-of-use and assay performance consistency. Oligo (dT) and random primers are pre-blended in a precise ratio to provide equal representation of 5′ and 3′-sequences for accurate gene expression quantification. The resulting cDNA product is directly compatible with all qPCR chemistries or conventional end-point RT-PCR of amplified fragments ≤1 kb in length.

For gene-specific priming (GSP) or two-step RT-PCR of RNA exceeding 1kb in total length, see QuantaBio’s qScript® Flex cDNA Kit.

Features & Benefits

  • Economical 2-component kit ideally suited for high throughput expression studies
  • Optimized, double pre-primed master mix component ensures equal representation of 5′ and 3′ RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR
  • Broad linear dynamic detection range with limiting (10pg) or plentiful (1ug) samples of total RNA
  • qScript cDNA Synthesis Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Contents

  • 5X concentrated master mix containing: titered primer blend (oligo dT(20) and random hexamer), qPCR-optimized dNTP blend and flexible magnesium titration
  • 20X concentrated qScript reverse transcriptase
  • Nuclease-free water

Storage & Handling

  • Store components in a constant temperature freezer at -25°C to -15°C upon receipt. After thawing, mix thoroughly before using.
  • For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
  • Thaw completely on ice then pulse vortex to mix and briefly spin-down to collect contents before opening tube. Concentrated reagents are viscous. When drawing out material, touch pipette tip to liquid interface but do not immerse.

Resources

Flyers

Product Manuals

Application Notes

CofA (PSFs)

SDSs

FAQs

    • Does the qScript™ cDNA Synthesis Kit include an RNase Inhibitor like the qScript™ cDNA SuperMix?

      Both kits contain an RNase inhibitor protein. qScript™ cDNA SuperMix is provided as a single tube reaction of 5X concentrated master mix. It provides all necessary components for first-strand synthesis including: buffer, dNTPs, MgCl2, primers, RNase inhibitor protein, qScript reverse transcriptase and stabilizers (except RNA template) . In the case of the qScript™ cDNA Synthesis Kit, Rnase inhibitor is premixed with the reverse transcriptase and is provided as a concentrated enzyme.

    • For first strand cDNA synthesis, is it better to use oligo(dT), random hexamers, gene specific primer (GSP) or combination of these primers?

      The choice of primer depends on your experimental goals. Oligo(dT) is recommended when using total RNA for cDNA synthesis. It is the key to full-length cDNA synthesis. Random hexamers give a series of short first-strand products spanning the entire mRNA. Use of random hexamers may be helpful if the PCR fragment is at the 5´ end of a large mRNA. To ensure full-length cDNA synthesis of large transcripts, oligo(dT) can be added along with random hexamers during first-strand synthesis. Gene specific primers (GSP) for cDNA synthesis may also be used and are required in a few applications such as 5´ RACE and qRT-PCR. For GC-rich templates, or templates rich in secondary structure, a GSP may not work as well as priming with oligo dT for first strand synthesis. If an RT-PCR is problematic, trying different options of oligo dT, random primers and/or GSP for priming first strand synthesis may find a solution. Oligo(dT)20 primer (Cat. No. 18418-020) is recommended for use with SuperScript III Reverse Transcriptase

    • What is the smallest quantity of RNA detectable by the qScript™ cDNA Synthesis Kit?

      Lower limits of detection by the qScript™ cDNA Synthesis Kit are dependent on many factors, such as primer design, target size, and the abundance of message. However, this system was able to detect GAPDH mRNA from as little as 1.0 pg of total HeLa RNA when used in conjunction with AccuStart Taq DNA Polymerase.

    • What is the RNA input for the qScript cDNA Synthesis Kit?

      The qScript cDNA Synthesis Kit provides for the quantitative conversion of 1µg to 10 pg total RNA to cDNA, with a reaction volume of 20 ul.

Publications

Fertility restoration in mice with chemotherapy induced ovarian failure using differentiated iPSCs
Kevin M. Elias – 2023
Glycopeptide-Based Supramolecular Hydrogels Induce Differentiation of Adipose Stem Cells into Neural Lineages
Vânia I.B. Castro – 2023
The plant cytosolic m6 A RNA methylome stabilizes photosynthesis in the cold
Vicente, A.M., Manavski – 2023
An Efficient Humanized Mouse Model for Oral Anti-Retroviral Administration
Amber K. Virdi – 2023
Sea transfer and net pen cleaning induce changes in stress-related gene expression in commercial Atlantic salmon (Salmo salar) gill tissue
Kine Samset Hoem – 2023

You may also like

    • qScript Ultra Flex Kit

      qScript Ultra Flex Kit

      QuantaBio

      qScript Ultra Flex Kit is easy-to-use and highly efficient for the synthesis of first-strand cDNA with flexible priming methods including oligo-dT, random primers and gene specific priming to reverse transcribe RNA to cDNA.

      Log in for pricing
    • qScript Ultra SuperMix

      qScript Ultra SuperMix

      95217 | QuantaBio

      Faster, better, tougher next generation cDNA SuperMix for first strand cDNA synthesis.

      Log in for pricing
    • qScript XLT cDNA SuperMix

      qScript XLT cDNA SuperMix

      QuantaBio

      qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved yield and stability at higher temperatures.

      Log in for pricing
    • qScript Flex cDNA Kit

      qScript Flex cDNA Kit

      QuantaBio

      The qScript Flex cDNA Synthesis Kit is configured in separate components for supporting multiple RT priming strategies. The kit provides optimized, flexible components for priming with oligo-dT, random hexamers, gene-specific primer (GSP), or any combination thereof.

      Log in for pricing
wp_reset_postdata();