qScript XLT cDNA SuperMix

Features

• Stabilized 5X concentrated master mix simplifies reaction assembly and improves accuracy by minimizing pipetting steps
• Optimized, double pre-primed SuperMix provides equal representation of 5′ and 3′ RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR
• Improved RNA template binding affinity for increased yields with problematic sequences or complex secondary structure
• Perform more qPCR assays from limiting amounts of RNA with 2-3 fold improved cDNA yields over older enzyme technologies
• Broad linear dynamic detection range spanning up to 108 fold

qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved yield and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than our previous qScript cDNA SuperMix cDNA synthesis kits, which utilize an engineered RNase H(+) reverse transcriptase mutant.

Contents
Single-tube, 5X concentrated reagent containing:

• Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP\
• Recombinant ribonuclease inhibitor protein (RIP)
• qScript XLT reverse transcriptase
• Titrated concentrations of random hexamer and oligo(dT) primer
• Proprietary enzyme stabilizers and performance-enhancing additives

Storage & Handling
Store components in a constant temperature freezer at -25°C to -15°C upon receipt
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Thaw completely on ice then pulse vortex to mix and briefly spin-down to collect contents before opening tube. 5X concentrated reagent is viscous. When drawing out material, touch pipette tip to liquid interface but do not immerse.

qScript XLT cDNA SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.