repliQa HiFi ToughMix

Features

• High Fidelity – >90x wild type Taq
• Extreme Speed – up to 3x faster PCR results with extension rates as fast as 1 kb/sec*
• Tough Tested – tolerant to a wide range of PCR inhibitors
• Superior Sensitivity – higher yields from lower inputs as little as 2 pg
• Long Range – amplify +24 kb gDNA, +40 kb λDNA

*for fragments less than 1 kb in size

repliQa HiFi ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

The repliQa HiFi ToughMix is a 2x, ready-to-use master mix that contains all the components for high fidelity PCR amplification, including a genetically modified DNA polymerase coupled with hot start antibodies. This unique, next generation master mix provides 90x higher fidelity compared to Taq, while reducing time to PCR results by 2-3x. The extreme speed is enabled by extension times as fast as 1-10 kb/sec depending on target length. The enzyme is coupled with the industry leading ToughMix which is tolerant to a wide variety of inhibitors making it suitable for routine PCR, cloning, amplicon sequencing and site directed mutagenesis.

Contents
2x reaction buffer containing optimized concentrations of MgCl2, dNTP’s and proprietarily formulated HiFi polymerase, hot start antibodies and ToughMix chemistry

Storage & Handling
Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.