RNase-Free DNase I

RNase-Free DNase I, 1 U/µL, 5000 Units
DNase I with no contaminating RNase activity.

  • Degrade dsDNA and ssDNA without damaging important RNA in your sample or reaction
  • Digest away unwanted DNA without digesting RNA
  • Use in a variety of applications such as in vitro transcription and RT-PCR

Includes 10X Reaction Buffer.

Name SKU Size Availability Vendor Price Order
RNase-Free DNase I D9905K 5,000 U @ 1 U/μl Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Product information
RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation. It efficiently hydrolyses dsDNA and ssDNA into a mixture of short oligonucleotides and mononucleotides.

Applications

  • Elimination of template DNA following in vitro synthesis of RNA with T7, SP6, or T3 RNA polymerase.
  • Labeling of DNA by nick translation, in combination with Klenow or other DNA polymerases.
  • Treatment of RNA prior to RT-PCR. 1
  • Characterisation of DNA-protein interactions by DNase I footprinting. 2,3

Epicentre-dnase_1

Figure 1. DNA removal from in vitro transcription reactions using RNase-Free DNase I. A linearised DNA template was transcribed using T7 RNA polymerase according to standard in vitro transcription conditions. Lane 1, kb ladder; Lane 2, DNA control; Lane 3, transcription mixture; Lane 4, transcription mixture treated with 1 MBU of RNase-Free DNase I for 15 minutes at 37 °C.
References

  1. Kienzle, N. et al. (1996) BioTechniques 20, 612.
  2. Sambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.) Cold Spring Harbor Laboratory Press, New York.
  3. Galas, D.J. and Schmitz, A. (1978) Nucleic Acids Res. 5, 3157.

Product specifications and usage

Unit Definition: One Molecular Biology Unit (MBU) of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutes at 37 °C under standard assay conditions.
Storage Buffer: 50% glycerol containing 10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, and 10 mM MgCl2.
DNase I 10X Reaction Buffer: 100 mM Tris-HCl (pH 7.5), 25 mM MgCl2, and 5 mM CaCl2.
5X Transcription Buffer: 0.2 M Tris-HCl (pH 7.5), 50 mM NaCl, 30 mM MgCl2, and 10 mM spermidine.

Resources

SDS

Manuals and User Guides

Manuals and User Guides

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