RNaseReveal™ Activity Assay Kit

Features

The RNaseReveal™ Activity Assay Kit is a fluorescent assay for the detection of RNase activity in liquid samples. The buffer and substrate have been formulated for maximum sensitivity, making it a useful tool for detecting RNase contamination in quality control workflows. Biotium also offers RNase A (Cat. No. 99871-600uL) as a separate product that is recommended for use with this kit.

Features

• Exceptional sensitivity, as little as ~0.08 pg RNase
• Simple 40 minute assay
• Detect with handheld fluorometer or fluorescence plate reader
• Useful for detecting RNase contamination in QC workflows

Kit components

• RNaseReveal Substrate™ (Cat. No. 31086A)
• 10X RNaseReveal™ Assay Buffer (Cat. No. 31086B)

The kit utilizes RNaseReveal™ Substrate which is an RNA probe tagged with a green fluorophore and a quencher, so that the intact probe is non-fluorescent. In the presence of RNase, the probe is cleaved and the fluorophore is detached from the quencher, releasing a green fluorescent signal. The lower limit of detection of RNase in the assay is ~ 0.08 pg, using Biotium’s RNase A (Cat. No. 99871-600uL) at ≥50 U/mg (Kunitz units). Full substrate cleavage is achieved with ~ 800 pg of the same RNase A. The excitation and emission maximum of the substrate is 496/516 nm. The substrate can be detected by any fluorescence plate reader or fluorometer capable of detecting green fluorescence. Biotium also offers RNase A (Cat. No. 99871-600uL) for use with this kit.