sparQ DNA Library Prep Kit

Features

• • Fast, easy single-tube solution completes library prep in 2.5 hours
  • Suitable for a wide range of input amounts from as low as 250 pg
  • Optimized chemistry ensuring superior library prep sensitivity and efficiency
  • Higher library yields compared to other library prep kits
  • High efficiency enables PCR-free workflow from 100 ng input

sparQ DNA Library Prep Kit is optimized for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms. The simplified protocol speeds up library prep to 2.5 hours with minimal hands-on time and accommodates DNA input amounts from 250 pg to 1 ug. DNA polishing reactions are streamlined into a single step to convert fragmented DNA into 5′-phosphorylated and 3′-dA-tailed DNA fragments. This is followed by high efficiency adapter ligation in the same tube. PCR-free workflows are enabled from 100 ng of starting material. If library amplification is required, the HiFi PCR Master Mix and Primer Mix ensure even amplification with minimal bias.

NGS Automation
Quantabio sparQ Frag & Library Prep Kit can be fully automated.
Currently sparQ Frag & Library Prep Kit is supported by the following Vendor Automation Platform(s)

• PerkinElmer Applied Genomics SCICLONE G3 NGSx
• Tecan DreamPrep NGS
• Beckman Coulter (in development)

sparQ DNA Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.