sparQ RNA-Seq HMR Kit

Ultra FAST RNA library prep with integrated rRNA & globin depletion.
Name | SKU | Size | Availability | Vendor | Price | Order | |
sparQ RNA-Seq HMR Kit – 8 Rxns |
95216-008 | 8 Rxns | Generally 1-2 weeks from receipt of order | QuantaBio | Log in for pricing | ||
sparQ RNA-Seq HMR Kit – 24 Rxns |
95216-024 | 24 Rxns | Generally 1-2 weeks from receipt of order | QuantaBio | Log in for pricing | ||
sparQ RNA-Seq HMR Kit – 96 Rxns |
95216-096 | 96 rxns | Generally 1-2 weeks from receipt of order | QuantaBio | Log in for pricing |
Features
sparQ RNA-Seq HMR Kit simultaneously depletes rRNA and globin mRNA while generating stranded RNA-seq libraries for Illumina® NGS platforms in 4.5 hours. In a single step and tube, RNA fragmentation and depletion of abundant ribosomal and globin transcripts (human, mouse, and rat) are integrated. The proprietary, highly optimized enzymes and streamlined workflow generate high quality, directional transcriptome NGS libraries from either intact or degraded RNA samples, with key improvements for low input and FFPE samples.
- High quality directional RNA library prep ready to sequence in a single day
- Simple workflow with 3 reaction tubes, 9 steps and 10 components
- Efficient and integrated removal of rRNA and globin mRNA from
human/mouse/rat (HMR) samples - Improved results for samples with limited quantity and/or poor quality RNA
Now with Bead Booster:
- Shorter total time investment of 4.5 hours for over 35% savings in time*
- Up to 5x increase in yield*
(*) compared to typical NGS RNA-seq with ribo-globin depletion
sparQ RNA-Seq HMR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Storage & Handling
Store the product in a constant temperature freezer at -25°C to -15°C upon receipt.
Once the ‘UDI Dilution Buffer’ has been thawed for use, it should be stored at 2 – 8°C.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Resources
Product Manuals
Application Notes
- Poster: Streamlined and high accuracy whole genome and transcription sequencing using Singular Genomics G4TM platform and Quantabio sparQ library prep kits
- Poster: Improved RNA-Seq Library Prep:Further simplifying whole transcriptome library prep using modifications to the sparQ RNA-Seq HMR Kit
- Poster: Simplified RNA-Seq Library Prep: Improved RNA sequencing results for FFPE and blood samples
- Application Note Streamlined Whole Transcriptome sequencing using Singular Genomics G4 platform and Quantabio sparQ RNA-Seq HMR Kit
- Rapid 16S Metagenomic Library Preparation for Oxford Nanopore Technologies (ONT)® Platform
- sparQ UDI Adapter sequence overview
- Illumina Experiment Manager version 1.9 files (zip-download)
- Illumina Experiment Manager version 1.8 files (zip-download)
- Setting up sparQ Library Prep Kit in Illumina Experiment Manager
Brochures
CofA (PSFs)
SDS’s:
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